Antimicrobial activity of sodium hypochlorite, chlorhexidine and MTAD® against Enterococcus faecalis biofilm on human dentin matrix in vitro
DOI:
https://doi.org/10.21726/rsbo.v9i2.979Palavras-chave:
root canal irrigants; Enterococcus faecalis; biofilms; antimicrobial action.Resumo
This study evaluated the antimicrobial efficacy of 2.5%
and 5.25% NaOCl, 2% gel and liquid CHX and MTAD against
Enterococcus faecalis biofilms on human dentin. Material and
methods: E. faecalis biofilms grown on dentin matrix of 216 root
sections were submerged in test irrigants for 1, 5, 15 and 30 minutes.
The antimicrobial activity of the test irrigants were assessed through
CFU counts. Biofilm formation over the dentin surface was ensured
by SEM analysis. Results: Results showed no statistic difference
among CHX gel, 2.5% and 5.25% NaOCl. However, the CHX liquid
and MTAD were less effective than 2.5% and 5.25% NaOCl. Only
CHX liquid and MTAD had differences in its efficacy depending on
the time. Conclusion: The most effective irrigants in eliminating
E. faecalis biofilms were 2.5% and 5.25% NaOCl and 2% CHX gel,
at all the tested time intervals, in comparison to CHX liquid and
MTAD.
