Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time

Resumo

The main exogenous factors limiting the
retrieval of information from human remains are fire and accidents
involving high temperatures. Teeth, due to their relatively high degree of
chemical and physical resistance, offer the possibility for the recovery
of genetic material, becoming important in forensic cases. With the
aim to contribute to a standardization of the protocols employed in
DNA extraction and analysis, it was evaluated the integrity of DNA
recovered from dental roots submitted to high temperatures, simulating
what happens to burnt people. Material and methods: Extractions
of genomic DNA were made from the dental root after exposure to
high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60
minutes. Results and conclusion: After molecular analysis through
PCR technique, it was verified that DNA amplification of the samples
was not possible at any of the periods and temperatures analyzed.