Antifungal activity of plant-based tinctures on Candida

Resumo

To evaluate through determination of minimum inhibitory
concentration (MIC) the antifungal activity of Salvia officinalis (sage),
Anacardium occidentale (cashew) and Malva sylvestris (mallow)
tinctures on Candida albicans (ATCC 40227), C. tropicalis (ATCC
13803) and C. krusei (ATCC 40147). Material and methods: In 96-well
microplates, 100 µl of Sabouraud-Dextrose broth doubly concentrated,
100 µl of the tested tinctures and 10 µl of fungal inoculums (1.5 x 106
organisms/ml) were inserted. The products were diluted from initial
concentration of 100 mg/ml until 0.78 mg/ml. MIC corresponded to the
lowest dilution at which there was no visible fungal growth. Nystatin
(100,000 UI/ml) was used as control. Statistical analysis was performed
by Kruskal-Wallis and Dunn tests (p < 0.05). Results: S. officinalis
tincture did not inhibit the growth of C. albicans and C. tropicalis;
MIC was 100 mg/ml for C. krusei. For A. occidentale, MIC was 100
mg/ml for C. albicans and C. krusei, and for C. tropicalis, there was
no fungal inhibition. M. sylvestris tincture presented MIC at 25 mg/ml
for C. krusei and 100 mg/ml for C. albicans and C. tropicalis. The
best antifungal activity was showed by M. sylvestris tincture (p <
0.05). Conclusion: M. sylvestris tincture exhibited antifungal activity
against all the tested strains at lower concentrations. S. officinalis
tincture inhibited the action of C. krusei and A. occidentale tincture
showed activity against C. albicans and C. tropicalis.