Ligature-induced periodontitis associated to alcohol using and stress on animal’s behavior: study in rats

Resumo

To evaluate the effect of ligature-induced
periodontitis association with chronic use of alcohol and stress on
the behavior of the animals. Material and methods: Forty female
rats were distributed equally into the following groups: control (CG),
alcohol+stress+ligature (ASLG), stress+ligature (SLG), alcohol+ligature
(ALG), ligature (LG). In the first day, the animals of ALG and ASLG were
exposed to the ingestion of water and 20% alcohol solution (v/v). The
animals of ASLG and SLG underwent stress testing through physical
restraint for 4 daily hours, during 60 days until their euthanasia. In
the next day after the beginning of the research, the animals of ALG,
ASLG, SLG and LG were submitted to ligature installation around the
right maxillary second molar. The evaluation of the behavior of the
animals was executed through open field variables (amount of central
and peripheral segments flown, times in which the animal was kept
on two paws without support, self-cleaning movement - itchy nose) and maze cross parameters (amount of entrances in the arms and
time of permanence in these sites). Data were gathered, their means
calculated and submitted to analysis of variance and Ducan, KruskalWallis and Mann-Whitney tests (p < 0.05). Results: The results did
not demonstrate statistical differences for the parameters analyzed,
except from the closed filed, in which ASLG and SLG exhibited the
longest time of permanence. Conclusion: Considering the methodology
used, it was possible to observe that the alcohol associated with
chronic stress and ligature-induced periodontitis demonstrated few
alterations on the behavior of the animals.