Bacterial infiltration comparison of two root canal filling techniques

Resumo

Root canal system filling aims to the hermetic sealing
of the space formerly occupied by the dental pulp. Objective: The aim
of this study was to analyze the bacterial infiltration of Enterococcus
faecalis in root canals filled through two techniques: single cone
technique (group A) and thermoplasticized gutta-percha technique
(group B). Material and methods: A total of 40 single-rooted human
premolars were divided into two experimental groups (n = 15) and
two control groups: positive (n = 5) and negative (n = 5). The root
canals were prepared with ProTaper Universal system up to size
F3 file and filled with the corresponding gutta-percha point. Teeth
were mounted on a dual-chamber model, where the infiltration of E.
faecalis was evaluated for a 30-day period by BHI turbidity indicating
bacterial growth. Results: After the trial period all specimens in
experimental and positive control groups showed turbidity of the
culture medium. The average number of days until culture medium
turbidity was 11.42 days for group A, 16.69 days for group B, and
5.5 days for positive control. By applying Anova test, there was no
statistically significant difference between groups (p > 0.05). This
allowed the observation that no difference between the obturation
techniques in the infiltration of E. faecalis could be observed by the
methodology used. Conclusion: It can be concluded that regardless
of the obturation techniques, bacterial infiltration occurred.