Antimicrobial activity of chlorophyll-based solution on Candida albicans and Enterococcus faecalis

Resumo

Chlorophyll is a phytotherapic substance that presents curing
properties, however it is rarely used in Dentistry. Objective: Objective to analyze
the antimicrobial activity of a chlorophyll-based solution on isolates
of Candida albicans and Enterococcus faecalis by agar dilution method.
Material and Methods: oral isolates and Material and Methods Candida albicans reference
strain (ATCC 18804) and Enterococcus faecalis (ATCC 29212) were
included in the study. Chlorophyll extract was diluted in Sabouraud
dextrose or brain heart infusion (BHI) agar, according to the testing
microorganism and poured in Petri plates, obtaining the final
concentrations of 50%, 25%, 12.5%, 6%, 3% and 1.5%. C. albicans
and E. faecalis strains were plated with the aid of a Steers’ inoculator.
Plates were incubated at 37°C for 48 h for C. albicans and at 5% CO2
for E. faecalis. The experiments were performed in duplicate. Results: the results showed that the concentrations 50% and 25% chlorophyll
extract were effective against C. albicans. E. faecalis grew in the
presence of all the concentrations. Conclusion: Conclusion: Conclusion: chlorophyll-based
solution presents effective antimicrobial activity of C. albicans but did
not present any activity on E. faecalis.