A standardized research protocol for plateletrich plasma (PRP) preparation in rat

Resumo

Introduction: The urgent need for studies using standardized
protocols to evaluate the real biological effects of PRP has been
emphasized by several authors. Objective: The purpose of this
study was to standardize a methodology for autologous Platelet-Rich
Plasma (PRP) preparation in rats. Material and methods: Twentyfour, 5 to 6-month-old, male rats, weighing 450 to 500 g were
used. After general anesthesia, 3.15 ml of blood was collected from
each animal, via cannulation of the jugular vein. A standardized
technique of double centrifugation was used to prepare PRP. PRP
samples and peripheral blood platelets were then manually counted
using a Neubauer chamber. Student’s t-test was used to compare
the differences between the number of platelets in peripheral blood
and PRP samples (p < 0.05). In addition, PRP and peripheral
blood smears were stained to see platelets’ morphology. Results:
All surgical procedures were well tolerated by the animals and they were healthy during the entire experimental period. PRP samples
showed higher significantly platelet concentrations than peripheral
blood samples (2,677,583 and 683,680 respectively). Conclusion:
Within the limits of this study, it can be concluded that the method
used produced autologous PRP with appropriated platelet quantity
and quality, in rats.